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lps from e. coli j5 strain  (List Biological Laboratories)

 
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    Structured Review

    List Biological Laboratories lps from e. coli j5 strain
    Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli <t>LPS</t> <t>(red),</t> <t>J5</t> E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown
    Lps From E. Coli J5 Strain, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lps+from+e%2E+coli+j5+strain/lps+from+e++coli+j5/pmc08294406-108-9-19
    Average 90 stars, based on 1 article reviews
    lps from e. coli j5 strain - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate"

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

    Journal: Journal of the American Society for Mass Spectrometry

    doi: 10.1007/s13361-018-1897-y

    Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli LPS (red), J5 E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown
    Figure Legend Snippet: Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli LPS (red), J5 E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown

    Techniques Used: Cell Culture, Incubation, Activation Assay

    Averaged IMS-CID tandem mass spectra of (a) J5 LPS m/z 1071 and (b) J5 dLPS m/z 789 after collision energy ramping. Insets show deprotonated fatty acid product ions’ presence in (a) and absence in (b)
    Figure Legend Snippet: Averaged IMS-CID tandem mass spectra of (a) J5 LPS m/z 1071 and (b) J5 dLPS m/z 789 after collision energy ramping. Insets show deprotonated fatty acid product ions’ presence in (a) and absence in (b)

    Techniques Used:

    Zoomed negative mode FT-ICR mass spectrum (R ~ 300,000 FWHM, in absorption mode) after direct infusion of J5 LPS. Eight potential isotopic distribution envelopes can be identified in absorption mode in this 4 m/z window; these are denoted, at the m/z of their respective monoisotopic ions, with blue arrows. (inset) Magnified portion of the spectrum showing fine detail (including the magnitude mode and the proposed overlap between isotopologues from envelopes 2 and 5)
    Figure Legend Snippet: Zoomed negative mode FT-ICR mass spectrum (R ~ 300,000 FWHM, in absorption mode) after direct infusion of J5 LPS. Eight potential isotopic distribution envelopes can be identified in absorption mode in this 4 m/z window; these are denoted, at the m/z of their respective monoisotopic ions, with blue arrows. (inset) Magnified portion of the spectrum showing fine detail (including the magnitude mode and the proposed overlap between isotopologues from envelopes 2 and 5)

    Techniques Used:

    MS3 CID mass spectra from MS2 product ions representing J5 E. coli lipid A at m/z 1796 (top) and core OS at m/z 1418 (bottom). Similar dissociation phenomena were observed as in MS2 experiments for chemically isolated lipid A and oligosaccharides, indicating feasibility of LPS top down sequencing in this manner
    Figure Legend Snippet: MS3 CID mass spectra from MS2 product ions representing J5 E. coli lipid A at m/z 1796 (top) and core OS at m/z 1418 (bottom). Similar dissociation phenomena were observed as in MS2 experiments for chemically isolated lipid A and oligosaccharides, indicating feasibility of LPS top down sequencing in this manner

    Techniques Used: Isolation, Sequencing

    Related Articles

    Purification:

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Materials Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Cell Culture:

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Materials Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Incubation:

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Materials Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Activation Assay:

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Materials Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Isolation:

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Materials Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Sequencing:

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Materials Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Gentle:

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Materials Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate
    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).



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    Image Search Results


    Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli LPS (red), J5 E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

    doi: 10.1007/s13361-018-1897-y

    Figure Lengend Snippet: Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli LPS (red), J5 E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown

    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Techniques: Cell Culture, Incubation, Activation Assay

    Averaged IMS-CID tandem mass spectra of (a) J5 LPS m/z 1071 and (b) J5 dLPS m/z 789 after collision energy ramping. Insets show deprotonated fatty acid product ions’ presence in (a) and absence in (b)

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

    doi: 10.1007/s13361-018-1897-y

    Figure Lengend Snippet: Averaged IMS-CID tandem mass spectra of (a) J5 LPS m/z 1071 and (b) J5 dLPS m/z 789 after collision energy ramping. Insets show deprotonated fatty acid product ions’ presence in (a) and absence in (b)

    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Techniques:

    Zoomed negative mode FT-ICR mass spectrum (R ~ 300,000 FWHM, in absorption mode) after direct infusion of J5 LPS. Eight potential isotopic distribution envelopes can be identified in absorption mode in this 4 m/z window; these are denoted, at the m/z of their respective monoisotopic ions, with blue arrows. (inset) Magnified portion of the spectrum showing fine detail (including the magnitude mode and the proposed overlap between isotopologues from envelopes 2 and 5)

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

    doi: 10.1007/s13361-018-1897-y

    Figure Lengend Snippet: Zoomed negative mode FT-ICR mass spectrum (R ~ 300,000 FWHM, in absorption mode) after direct infusion of J5 LPS. Eight potential isotopic distribution envelopes can be identified in absorption mode in this 4 m/z window; these are denoted, at the m/z of their respective monoisotopic ions, with blue arrows. (inset) Magnified portion of the spectrum showing fine detail (including the magnitude mode and the proposed overlap between isotopologues from envelopes 2 and 5)

    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Techniques:

    MS3 CID mass spectra from MS2 product ions representing J5 E. coli lipid A at m/z 1796 (top) and core OS at m/z 1418 (bottom). Similar dissociation phenomena were observed as in MS2 experiments for chemically isolated lipid A and oligosaccharides, indicating feasibility of LPS top down sequencing in this manner

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

    doi: 10.1007/s13361-018-1897-y

    Figure Lengend Snippet: MS3 CID mass spectra from MS2 product ions representing J5 E. coli lipid A at m/z 1796 (top) and core OS at m/z 1418 (bottom). Similar dissociation phenomena were observed as in MS2 experiments for chemically isolated lipid A and oligosaccharides, indicating feasibility of LPS top down sequencing in this manner

    Article Snippet: Purified (low protein and nucleic acid content) and lyophilized LPS from E. coli J5 strain was purchased from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO).

    Techniques: Isolation, Sequencing